CB 399 Microbial Genetics in Infectious Disease Spring 2010

Intellectual Unit:

Microbial Genetics in Infectious Disease

Course Lecturers: Deborah Hung, Eric Rubin, and Jim Gomez

Curriculum Fellow: Sarah Wojiski , sarah_wojiski@hms.harvard.edu

In this nanocourse, we will discuss the current state of technologies and approaches that are available to study bacterial behavior with an emphasis on physiology and pathogenesis as it applies to infectious diseases. Topics covered will include classical genetic, genomic and chemical biological approaches to understanding bacterial behavior, with the goal of highlighting the advantages and limitations of each of these methods, newer approaches on the horizon, and ongoing challenges. Examples will be drawn from the literature and placed within clinical context.

First Meeting: Tuesday, April 6, 1- 4:30 pm

Location: TMEC Bldg., room 250

Second Meeting: Monday, April 12, 2- 4:30 pm

Location: TMEC Blgd., room 448

Recommended Readings

Avery 0, MacLeod C, and McCarty M.  (1944) Studies on the chemical nature of the substance inducing transformation of Pneumococcal types.  J Exp Med 79:137-160.

Judson M and Mekalanos J.  (2000) Transposon-based approaches to identify essential bacterial genes.  Trends in Microbiology 8:521-526.

Stanley S and Hung D. (2009) Chemical tools for dissecting bacterial physiology and virulence.  Biochemistry 48:8776-8786.

Mahan M, Slauch J, and Mekalanos J. (1993) Selection of bacterial virulence genes that are specifically induced in host tissues. Science 259:686-688.

Sassetti C, Boyd D, and Rubin E. (2001) Comprehensive identification of conditionally essential genes in Mycobateria. PNAS 58:12712-12717.

Fortune S, Chase M, and Rubin E. (2006) Dividing oceans into pools: strategies for the global analysis of bacterial genes. Microbes and Infection 8:1631-1636.

Burrack L and Higgins D. (2007) Genomic approaches to understanding bacterial virulence. Curr Opin Microbiol 10:4-9.