CB 399: Single-Cell Sequencing: Experimental Design, Analysis, and Practical Applications

Single-Cell Sequencing: Experimental Design, Analysis, and Practical Applications

Single-cell sequencing technologies allow us to study genomes and transcriptomes at the single cell level. These relatively new techniques have expanded our knowledge in a wide range of fields from microbiology to development and cancer biology. This course will provide a general introduction to both RNA and DNA-based single-cell sequencing. We will start with a broad overview of RNA-sequencing in single cells with an emphasis on current and emerging technologies. Subsequent lectures will focus on experimental design for single-cell experiments and common approaches to data analysis.

Class Assignments
Registered participants will use the technologies, experimental design, and analysis methods presented in Session 1 to design a single-cell sequencing experiment. They will write a one-page specific aims proposal and give a 5-minute presentation of the experiment.

Course Instructors: Evan Macosko,emacosko@broadinstitute.org and Mandovi Chatterjee,Mandovi_Chatterjee@hms.harvard.edu
Course Director: Peter Kharchenko, Peter_Kharchenko@hms.harvard.edu
Curriculum Fellow: Rachel Wright, rachel_wright@hms.harvard.edu

Session Dates and Times
First Session Date: March 7, 2019 - 1:00pm - 4pm
First Session Location:TMEC 227
Second Session Date: March 14, 2019 - 1:00pm - 4pm
Second Session Location: For Registered participants only

Peter's analysis walkthrough:
http://pklab.med.harvard.edu/peterk/nano2019.html