CB 399:Single-Cell Sequencing: Experimental Design, Analysis, and Practical Applications

Single-Cell Sequencing: Experimental Design, Analysis, and Practical Applications

Single-cell sequencing technologies allow us to study genomes and transcriptomes at the single cell level. These relatively new techniques have expanded our knowledge in a wide range of fields from microbiology to development and cancer biology. This course will provide a general introduction to both RNA and DNA-based single-cell sequencing. We will start with a broad overview of RNA-sequencing in single cells with an emphasis on current and emerging technologies. Subsequent lectures will focus on experimental design for single-cell experiments and common approaches to data analysis. Our final lecture will review genomic sequencing in single cells

Class Assignments: Registered participants will use the technologies, experimental design, and analysis methods presented in Session 1 to design a single-cell sequencing experiment. They will write a one-page specific aims proposal and give a 5-minute presentation of the experiment.

Course Directors: Mandovi Chatterjee
Course Instructors: Navpreet Ranu, Alexa Goeva, Joshua Welch and Daniel Wagner
Curriculum Fellow: Rachel Wright, rachel_wright@hms.harvard.eduand Ted Feldman, theodore_feldman@hms.harvard.edu
 
Session Time and Dates
First Session: March 1, 2018, 11:00 - 2:00pm
First Session Location: Minot Room, Countway Library
Second Session: March 8, 2018, 11:00 - 2:00pm
Second Session Location: TMEC 104

AUDITORS (Post-Docs, Faculty, or Staff) DO NOT NEED TO SIGN UP TO ATTEND THE 1st SESSION. PLEASE DO NOT ENROLL.