CB 399: CRISPR-Cas Systems and the Future of Genome Editing

RISPR-Cas Systems and the Future of Genome Editing

Clustered regularly interspaced short palindromic repeat (CRISPR) RNAs and their CRISPR-associated (Cas) proteins are an important part of adaptive immune systems in many prokaryotes. CRISPR-Cas systems function as RNA-directed endonucleases that can target nucleic acids in a sequence-specific manner and are now widely used as genome editing tools. In this course, we will provide lectures covering: an introduction to genome editing and cutting-edge improvements to CRISPR-Cas systems; a review of bioinformatics tools for guide RNA design and analysis of CRISPR-Cas data; and an overview of ongoing and potential therapeutic applications of genome-editing nucleases. The course will also include a practical lab-based workshop for registered students in which participants will learn how to design guide RNAs and how to quantify nuclease-induced mutations in any cell or organism using sequencing-based assays.

Class Assignments: Complete analysis of a sequencing-based experiment designed to assess mutation frequency induced by a CRISPR-Cas nuclease at an endogenous human gene target site. Lecturers: Luca Pinello and Becca Cottman.

Course Instructors: Luca Pinello (lpinello@mgh.harvard.edu), John Doench(jdoench@broadinstiture.org) and Morgan Maeder(morganlmaeder@gmail.com)
Course Director: Keith Joung (jjoung@mgh.harvard.edu)
Curriculum Fellow: Rachel Wright, rachel_wright@hms.harvard.edu

Session Dates and Times
First Session: Tuesday, October 10 2017; 9:00 - 12:00pm
First Session Location: Armenise Amphitheater
Second Session Date: Tuesday, October 17 2017; 1:00 - 5:00pm
Second Session Location: Joung Lab - 149 13th Street, Charlestown, MA