CB 399: Single-Cell Sequencing: experimental design, analysis, and practical applications

Single-Cell Sequencing: experimental design, analysis, and practical applications

Course Description
Single-cell sequencing technologies allow us to study genomes and transcriptomes at the single cell level. These relatively new techniques have expanded our knowledge in a wide range of fields from microbiology to development and cancer biology. This course will provide a general introduction to both RNA and DNA-based single-cell sequencing. We will start with a broad overview of RNA-sequencing in single cells with an emphasis on current and emerging technologies. Subsequent lectures will focus on experimental design for single-cell experiments and common approaches to data analysis. Our final lecture will review genomic sequencing in single cells.
This nanocourse is sponsored by the Program in Genetics and Genomics and the Landry Cancer Biology Consortium.
Nanocourse Director: Peter Kharchenko
Curriculum Fellows: Ted Feldman(theodore_feldman@hms.harvard.edu) Emily Gleason (emily_gleason@hms.harvard.edu) and Meg Mittelstadt (megan_mittelstadt@hms.harvard.edu)

Lecturers
• Daniel Wagner, Postdoctoral Research Fellow, Klein Lab, Harvard Medical School
• Rafael Irizarry, Professor of Biostatistics, Harvard T.H. Chan School of Public Health
• Peter Kharchenko, Assistant Professor of Biomedical Informatics, Harvard Medical School
• Paul Blainey, Assistant Professor of Biological Engineering, Massachusetts Institute of Technology and The Broad Institute.

Session Dates and Times
Session 1: Lectures (open to all without prior registration)
Date: Tuesday, March 7th 9:00 am to 1:00 pm
Location: Armenise 125

Session 2: Discussion (limited to registered students)
Date: Tuesday, March 14th 10:00 am to 12:00 pm
Location: TMEC 447

Assignment
TBA

AUDITORS (Post-Docs, Faculty, or Staff) DO NOT NEED TO SIGN UP TO ATTEND THE 1st SESSION. PLEASE DO NOT ENROLL.