CBB 399: Metabolomics: Utilizing Metabolomic Data in Scientific Research

Metabolomics: Utilizing Metabolomic Data in Scientific Research

Course Instructors: Kerry Ivey, BSc, P.Grad. Dip, PhD (kivey@hsph.harvard.edu); Rachel Kelly, BSc (Hons) MPH PhD (hprke@channing.harvard.edu)

Curriculum Fellow: Bradley Coleman, bradley_coleman@hms.harvard.edu
Course Director: Eric Rubin MD, PhD

Metabolomics, an interdisciplinary “omics” science, that combines bioinformatics, epidemiology, analytical biochemistry and biology, may represent the ‘final frontier’ in biomarker development.

Metabolomics is the agnostic study of all the low molecular weight compounds in a biological sample to provide a dynamic down-stream measure of a whole system’s activity defined as the metabolome; a global measure analogous to the genome. The metabolome reflects the genome, epigenome, transcriptome and proteome. It also reflects current exposure experience such as diet.

Crucially to public health, the metabolome has been shown to be reflective of disease state. As such, it represents a rich resource for the identification of exposure, predictive, diagnostic and prognostic biomarkers. With the advancement of high-throughput technologies, metabolomics is now emerging as a powerful and unique tool that will make a substantial impact on discovery-driven science.

In this nanocourse, we will provide an overview of metabolomics, the concepts and technologies underlying it, and recent developments in the field. We will provide the basic framework for planning and executing successful metabolomic studies, enabling nanocourse participants to take advantage of this exciting new technology in their future work.

Learning Objectives - Upon completion of this nanocourse, participants will be able to:

DAY 1:

Part 1
• Define metabolomics and understand the concepts underlying this methodology
• Describe the different technologies used to conduct metabolomic profiling
• Review the necessary components and criteria for conducting a metabolomics study
Part 2

• Analyze and interpret metabolomic data
• Compare the role of metabolomics in the study of environmental exposure and in the study of health and disease
Part 3
• Discuss how to maximize the utility of metabolomics in successful grant applications
DAY 2:
• Gain hands-on experience in critiquing real-world metabolomic case-studies
• Prior to the class attendees will receive case-studies; during the class the design, methodology and results of these studies will be broken down and appraised, working both in small groups and as a class. By the end of the session attendees will have a thorough understanding of the entire metabolomics pipeline from conception to publication and translation, and the ability to critiquely interpret the findings of metabolomics studies throughout the scientific literature
First Session: Thursday October 6th 12.30pm-3.30pm
Location: KRESGE G1

Second Session: Friday October 7th, 12.30pm- 3:30 PM
Location: KRESGE G2
Attendance at session two is a requirement to obtain academic credit but is open to all

**This course is suitable for those who attended last year’s Metabolomics Nanaocourse and for those completely new to the field**