CB 399: Introductory Flow Cytometry Fall 2015

Introductory Flow Cytometry

Course Director: Dr. Tim Mitchison

Curriculum Fellow:  Kevin Bonham (kevin_bonham@hms.harvard.edu) and Emily Gleason
Lecturer: Dr. Jodene Moore, Director of the HMS Systems Biology Flow Cytometry Facility  

Flow cytometry is a technology that can be used for a wide range of both clinical and research-based applications. It offers qualitative and quantitative data, describing cellular phenotypes and functional properties.  Recent advances in hardware, instrumentation and reagents have now made it possible to use higher-order polychromatic assays of complex design that measure up to 27 parameters. These multicolor assays offer great potential to ask new questions, or provide new answers to old questions. This nanocourse will introduce you to the subsystems of the instrument, measurements in flow cytometry and some of the complications that are prevalent in this technique. In addition, the course will cover sample preparation and give brief examples of common applications including sorting.

Schedule: 

First Session (open to the Harvard community): Monday, October 5th 12:30 – 4 PM
Location: Systems Biology Conference Room - Armenise 506

Second Session (limited to registered students): Wednesday, October 14th 12:30 – 3 PM
Location: Countway Library, Ballard Room

First Session Schedule:

- Introduction to Flow Cytometry

- Flow Cytometer Subsystems:

  • Fluidics
  • Optics
  • Electronics

-  Measurements in Flow Cytometry

-  Spectral Bleed/Compensation

BREAK-15 minutes

-  Core Instrumentation/Configurations

-  Antibodies/Epitopes

-  Sample Prep/Applications

-  Mass Cytometry

Assignment: Propose a hypothesis related to your current field of study that could be tested using flow cytometry. Please provide both your hypothesis and a rationale and discuss other experimental options that could be utilized to prove or disprove your hypothesis. Please email your assignments to Kevin Bonham (kevin_bonham@hms.harvard.edu) by 5pm Sunday, October 11th. Kevin will compile all of the assignments and share them with the class. Please come to the second session prepared to discuss your hypothesis and those of your classmates and bring any questions you might have about flow cytometry.

DROP DEADLINE: Monday, September 28, 2015

 AUDITORS (Post-Docs, Faculty, or Staff) DO NOT NEED TO SIGN UP TO ATTEND THE 1st SESSION.  PLEASE DO NOT ENROLL