CB 399: Next Generation Sequencing Technologies: Principles and Applications Fall 2009

Intellectual Unit: Experimental Tools for Biological Discovery

Next Generation Sequencing Technologies: Principles and Applications

Course Director: Chad Nusbaum
Course Lecturers: Mark Daley, Gabor Marth and Chad Nusbaum

Curriculum Fellow: Dr. Sarah Wojiski

Traditional capillary sequencing technology using base-specific chain termination by fluorescent di-deoxy nucleotides represents modifications to the original sequencing methodology devised by Sanger and colleagues in the 1970s.  Recent years have seen the  development of next generation parallel sequencing technologies that are rapidly replacing older methodologies.  Sequencing by synthesis enables the simultaneous sequence analysis of millions of DNA templates at the same time, or in parallel.  These new approaches allow for DNA sequencing at a markedly faster pace, and often at a much cheaper price, making sequencing projects feasible for an ever-expanding number of researchers.  This nanocourse will explore the methodology and principles behind parallel sequencing technology, and how it measures up to traditional sequencing methods.  Examples of the numerous applications of this ever-evolving technology, as well as the limitations of parallel sequencing, will also be discussed.

First Meeting: Wednesday, October 7th, 9am-12:30pm

Location: TMEC Bldg., Room 250

Second Meeting: Friday, October 9th, 1-3:30pm

Location: TMEC Bldg., Room 109

Recommended Readings

Instructions and Course Documents