CB 399: Next Generation Sequencing Technologies: Principles and Applications Fall 2013

Next Generation Sequencing Technologies:  Principles and Applications
Nanocourse Director: Dr. Fred Winston
Curriculum Fellow: Emily Gleason, Emily_gleason@hms.harvard.edu

Lecturers:

  • Dr. Chad Nusbaum, Co-director, Genome Sequencing and Analysis program, Broad Institute
  • Dr. Peter Park, Associate Professor of Pediatrics, Children’s Hospital, and HMS Center for Biomedical Informatics
  • Mr. Robert Steen, Director, Harvard Medical School Biopolymers Facility

 

Traditional capillary sequencing technology using base-specific chain termination by fluorescent di-deoxy nucleotides represents modifications to the original sequencing methodology devised by Sanger and colleagues in the 1970s.  Recent years have seen the development of next generation parallel sequencing technologies that are rapidly replacing older methodologies.  Sequencing by synthesis enables the simultaneous sequence analysis of millions of DNA templates at the same time, or in parallel.  These new approaches allow for DNA sequencing at a markedly faster pace, and often at a much cheaper price, making sequencing projects feasible for an ever-expanding number of researchers.  This nanocourse will explore the methodology and principles behind parallel sequencing technology, and how it measures up to traditional sequencing methods.  A discussion of the services available at the Department of Genetics Biopolymers core facility, including order placement, data output, and turnaround times, will also be included for researchers interested in utilizing these resources.

Schedule:

First Session: Thursday, December 5th, 9:00 a.m. – 12:30 p.m.
Location: TMEC Building, Walter Amphitheater

Videos: Part 1   Part 2   Part 3

Second Session: Thursday, December 12th, 1:00 p.m. – 3:30 p.m.
Location: TMEC Building, Room 447

 

 DROP DEADLINE: Wednesday, November 27, 2013

 

Assignment: 

Registered students should prepare a one-page document detailing a research question that could be at least partially answered by next-generation sequencing methods, and how you would propose to use them.  Please send your documents (either Word files or pdf files) to Emily Gleason (Emily_Gleason@hms.harvard.edu) by 5:00 p.m. on Tuesday, December 10th.  Your proposals will be sent to the course lecturers prior to the session so they can look them over and prepare to discuss them with you.  Please bring a copy of your proposal to the session as well for your own reference. A recommended reading list is provided below to help you with your proposal. 

 

AUDITORS (Post-Docs, Faculty, or Staff) DO NOT NEED TO SIGN UP TO ATTEND THE 1st SESSION.  PLEASE DO NOT ENROLL