CB 399: 2-Photon Microscopy: Past, Present and Future Fall 2009

Intellectual Unit: Experimental Tools Discovery for Biological

2-Photon Microscopy: Past, Present and Future

Course Director: Sean Megason

Course Lecturers: Sean Megason, Thorsten Mempel and Clay Reid

Curriculum Fellow: Dr. Anupama Seshan

2-photon fluorescence microscopy utilizes highly focused photons of light in order to image biological processes deep within a tissue. Since multiple, low energy wavelengths of light are used for excitation, 2-photon microscopy reduces the phototoxicity and light scattering that are unavoidable in other types of fluorescence microscopy. Ultimately, the development of 2-photon microscopy has allowed scientists to study biological processes in a more ‘natural’ setting by imaging in whole mount preparations and even in live, whole animals. 2-photon microscopy was first described in 1990, and its applications and utilization have been expanded ever since. In this nanocourse, lecturers will review the basic theory that makes 2-photon microscopy so useful and describe how they utilize the technology in their own research. We will end the nanocourse with lecturer’s own predictions on the future use of multiphoton microscopy technology.

First Meeting: Wednesday, September 30, 1 pm - 4:30 pm

Location: Cannon Room

Second Meeting: Friday, October 2, 2 pm - 5 pm

Location: Armenise 5th floor conference room

Recommended Readings

Instructions and Course Documents