CB 399: Fluorescence Live Cell Microscopy Fall 2011

 Intellectual Unit:

Fluorescence Live Cell Microscopy
Nanocourse Director: Jennifer Waters
Curriculum Fellow: Ondra Kielbasa

Fluorescence microscopy has become increasingly important in biological research as novel fluorescent probes and increasingly sensitive detectors allow us to visualize subcellular entities in live specimens with unprecedented speed and resolution. The aim of this practical course is to introduce students to the wide range of fluorescent probes and fluorescence microscopy techniques available for imaging live specimens (including optical sectioning techniques such as confocal and total internal reflection fluorescence).

Schedule:

First Session: Lecture, Tuesday, September 27, 2011 1 - 4:30 pm   
Location: Building C, Cannon Room
 
Second Session: Laboratory, Friday, September 30, 2011 1 – 5 pm
Location: LHRRB 113, Nikon Imaging Facility

Please note:  Students that are interested in the laboratory section should sign up on the website.  However space in the laboratory session is limited and signing up on the website will not guarantee a spot in the session. To be considered for the laboratory section of this course, students should also submit a statement of interest (three sentences at the most).  In this statement, please say whether you have any experience with fluorescence live imaging (and if so, how much experience) and also why you would like to take the second session for this nanocourse.  These should be emailed to the curriculum fellow (Ondra Kielbasa, ondra_kielbasa@hms.harvard.edu) no later than September 13th.  They will be reviewed by the course director, Dr. Jennifer Waters.  Students will then be notified by email regarding whether or not they will be able to attend the laboratory session.

Readings:

Registered students should complete the following readings prior to the first session (these can be downloaded from the attachment box below - you must be logged into the website to view and download the PDF files).  In addition to the four readings, students taking the lab session should review the laboratory protocol (click on the file named "Waters Lab 2011" in the attachments box below).

Readings:

Murray JM, Appleton PL, Swedlo JR, and Waters JC. 2007. Evaluating performance in three-dimensional fluorescence microscopy.  Journal of Microscopy.  228: 390–405

North AJ. 2006.  Seeing is believing? A beginners' guide to practical pitfalls in image acquisition. J Cell Biol. 172: 9-18.

Shaner NC, Steinbach PA, and Tsien RY.  2005. A Guide to Choosing Fluorescent Proteins.  Nature Methods. 2: 905-909.

Waters JC. Chapter 7. Live-Cell Fluorescence Imaging.  In Methods in Cell Biology. Vol. 81. Copyright 2007, Elsevier Inc.

 

 

DROP DEADLINE: Tuesday, September 20, 2011

 

AUDITORS (Post-Docs, Faculty, or Staff) DO NOT NEED TO SIGN UP TO ATTEND THE 1st SESSION.  PLEASE DO NOT ENROLL.