CB 399: Biosensors Nanocourse Spring 2011

Intellectual Unit:

Biosensors Nanocourse

Course Lecturers: Jessica Tytell (HMS Cell Biology), Gary Yellen (HMS Neurobiology), Barbara Imperiali (MIT Dept. of Chemistry)

Curriculum fellow: Ondra Kielbasa, Ondra_Kielbasa@hms.harvard.edu 

The goal of this course is to introduce the design principles and applications of fluorescence-based biosensors in cell biology.  First, an overview of biosensors will be given to introduce general concepts and usage of fluorescent-protein based systems and environment sensitive dye-based systems. The second lecture will describe the design, execution and analysis of a protein-based biosensor. The third lecture will introduce different chemistries and design strategies for environmentally-sensitive chemical probes and dyes. The combination of these lectures is designed to introduce students to the basic types of biosensors and insight into their design.  This nanocourse is meant to be a broad introduction to the concepts of biosensor design and application and to encourage students to think about the ways in which biosensors can be utilized to ask deeper questions about their research. It is not intended to serve as a technical introduction to biosensor work and therefore, although we will cover the need for controls and what types of controls should be done in various cases, we do not intend to give extensive technical detail about imaging conditions or analysis methods.

Schedule:

First Session: Tuesday, March 29, 2011, 1 – 4:30 pm (note: this session is open to all)
Location: Goldenson 122

Second Session: Thursday, April 14, 2011, 1 – 4 pm (note: this session is for registered students only)
Location: TMEC Building, Room 309

Lectures:

You can download PDF files of the lectures from the attachments box below.

Lecture 1 -  Introduction to Biosensors: Design and Usage

Lecture 2  - Protein Biosensor Design

Lecture 3 - The Expanding Chemical Space of Organic Fluorphores: Development and Applications

Readings:

Galen S. Loving, Matthieu Sainlos, and Barbara Imperiali.  2009. Monitoring protein interactions and dynamics with solvatochromic fluorophores. Trends in Biotechnology.  28: 73-83. (download from the attachments box below)

 

DROP DEADLINE: Tuesday, March 22, 2011

 

AUDITORS (Post-Docs, Faculty, or Staff) DO NOT NEED TO SIGN UP TO ATTEND THE 1st SESSION.  PLEASE DO NOT ENROLL.