CB 399 Microfluidics Spring 2010

Intellectual Unit:

Microfluidics

Course Lecturers: Jagesh V. Shah, Daniel Irimia, Mehmet Toner, Fettah Kosar
Lab Coordinators: Natalie Andrew, Dan Huh, Victor Lien

Curriculum Fellow: Anu Seshan, Anupama_Seshan@hms.harvard.edu

Microfluidics is a multidisciplinary field that intersects biology, engineering, physics, and chemistry. This field, which deals with the precise control and manipulation of fluids that are geometrically constrained to a small volume, has evolved considerably in the last three decades since its inception. Advances in microfluidics technology are revolutionizing procedures for DNA analysis, enzymatic assays and proteomics. An exciting emerging application is at the interface with cells and organisms, where microfluidics-based devices are being used to create controlled microenvironments in which behavior can be observed and manipulated. In this nanocourse, which will begin with a description of the principles of microfluidics, you will learn about the fabrication of basic devices and their many uses in understanding complex biological problems. On day two, you will then have the opportunity to walk through a microfluidics experiment from start to finish and create your own microfluidic chip in the lab.

First Meeting: Tuesday, April 13, 2010 1:30pm to 5:00pm ,

Location: Goldenson 122

Second Meeting:  Thursday, April 15, 2010 1:00pm to 6:00pm,

Location:  Microfluidics facility (students will sign up for one of three 3 hour time slots starting at 1pm, 2pm, or 3pm).

Limited to 12 students.

Lecture slides and video

Please download the lecture slides from the nanocourse below.

Click here to view the lectures.

Assigned Readings

Please read the following review articles prior to lecture on April 13, 2010 (which can can be downloaded by clicking on the link at the bottom of the page):
1) Young EWK and Beebe J. 2010. Fundamentals of microfluidic cell culture in controlled microenvironments. Chem. Soc. Rev. 39: 1036-1048.
2) El-Ali et al. 2006. Cells on chips. Nature 442: 403-411.
3) Whitesides G. 2006. The origins and the future of microfluidics. Nature 442: 368-373

Please carefully read over the protocol for the lab component of the course before lab on April 15th, 2010, which is posted below.

Please Enroll/Un-enroll for this nanocourse below